Carnivorous Plants Website
Carnivorous Plants in the Wilderness
by Makoto Honda

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Summer of 2018
Venus Flytrap Seed Sowing / Germination

2018-07-28  (Day 1) - Venus Flytrap
Venus flytrap seeds sowed on Sphagnum moss (sterilized by boiling water)

2018-08-11 (Day 13)
One seed germinated...  Venus Flytrap

2018-08-20 (Day 21)
More on the way...   Venus Flytrap

Venus Flytrap Germination

2018-08-21 (Day 22) - Venus Flytrap


2018-08-29 (Day 30) - Venus Flytrap

Venus Flytrap Germination

2018-09-06 (Day 38 - since seed sowing) - Venus Flytrap

2018-September-06.  Venus Flytrap Germination Photo:  4-week old seedling  (Copyright 2018 Makoto Honda. All Rights Reserved.)

 Nikon D300, Macro Topcor 58mm F3.5 with extension, at f/8 at 1/30 sec (daylight), ISO 200. Photo processing by Nikon Capture NX.
To reduce the possible camera movement, I used 5-sec self-timer and 2-sec shutter delay mode.
Also, with this magnification, the lens aperture beyond f/8 or f/11 will result in noticeable loss of resolution (due to diffraction).

 


2018-September-07.  Venus Flytrap Germination Photo:  4-week old seedling  (Copyright 2018 Makoto Honda. All Rights Reserved.)
 

 


2018-September-07.  Venus Flytrap Germination - Baby Trap.
 

 


2018-September-07.  Venus Flytrap Germination - Trigger hairs & digestive glands on the very first trap of this Venus flytrap seedling.

 


2018-September-07.  Venus Flytrap Germination - Trigger hairs & digestive glands on the very first trap of this Venus flytrap seedling.
I see four trigger hairs. There are about 40-50 digestive glands on each lobe. This baby trap is about 2mm long.

 


2018-September-08.  Venus Flytrap Germination - Trigger hairs & digestive glands on the very first trap of this Venus flytrap seedling.
 

 


2018-September-08.  Venus Flytrap Germination - Trigger hairs & digestive glands on the very first trap of this Venus flytrap seedling.
I see two trigger hairs on each lobe.
 


2018-September-09.  Venus Flytrap Germination - Trigger hairs & digestive glands & Nectaries of the very first trap of a Venus flytrap seedling.
Nikon D300, Macro Topcor 58mm f3.5, at f/8, 1/20 sec. Natural light. ISO 200. The trap is about 2mm long.
 


2018-September-10.  Venus Flytrap Baby - Snap-trap.

 


2018-September-10.  Venus Flytrap Baby - Snapping on trigger hair stimulation by a needle.

 


2018-September-13.  Venus Flytrap Germination

2018-09-13 : One more seed germinated - this is after 45 days of seed sowing on the sphagnum moss.
I had about 25 seeds - close to 20 germinated so far.
 


2018-September-20.  Venus Flytrap Germination
The third trap is now opening...

2018-September-10.  Venus Flytrap Germination - The first trap

(Image stacking by Photoshop 2018)

 


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Venus Flytrap Seed Germination

2018-7-00  VF seed ordered (x20)
2018-7-28  Sowing on wet sphagnum moss
2018-8-11  First one seed germinated
2018-8-29  15 germinated so far...
2018-9-07  The very first trap formed
2018-9-15  The last germination - Total 16 out of 25
2018-9-16  One plant now has 2 traps open
2018-9-20  Last day of trap photo shoot -  done
 

Technical Notes

Camera used: Nikon D300
Lens: Macro Topcor 58mm F3.5 (no reverse ring used)
Lens used in normal direction to use its helicoids ring (very important for fine-focus)
Lighting: Daylight (sun light)
ISO: 200
Shutter settings:
   Self-timer 5 sec.
   Exposure delay mode 2 sec.
   Block viewfinder with plastic cover (important)
Shutter speed: 1/90, 1/40, 1/20, 1/10
Aperture:  F5.6, F8, F11, F16 (F22 not used)
Best apertures: F5.6 / F8 / F11 / F16 (F8, F11 best)

F11 is used for final shots.

Focus stacking shots made by F8.


PhotoShop 2018 Focus Stacking

 Jump into PhotoShop

File --> Script --> LOad files into stack...

Load Layers panel
   use: Files --> Files --> Browse
   Go to my folder and select ALL frames --> OK

   Click - Attempt to automatically align source images
   Unclick - Create smart object

   --> OK

Now,right pane, select all layers by clicking 1st & shift+click last

Then,

Edit --> Auto Blend Layers ...

Auto Blend Layers panel
   Click - Stack Images
   Click - Seamless tones and colors

   ---> OK

Takes some time to finish

Then save as ...

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